Mahmoud Kandeel1,2,*, Abdulhafez Dalab3, Abdulkarem Al-Shabebi3, Abdulla Al-Taher1, Yousef Altaher1 and Mohamed Abozahra1 1Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Alahsa, 31982, Saudi Arabia 2Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 35255, Egypt 3Department of Anatomy, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Alahsa 31982, Saudi Arabia
ABSTRACT
In this work, the pyrimidine metabolic pathway Uridine 5’- Triphosphate (UTP) paths were investigated. In this context, two UTP enzymes were investigated by bioinformatics tools, nucleoside diphosphate kinase (NDK) and cytidine triphosphate (CTP) synthase. The dromedary, Bactrian and feral camels NDK showed high similarity > 97.7% to the human enzyme. The camel NDK was phylogenetically distant from eukaryotic NDK with the closest relation to prokaryotic NDK. Trypanosoma evansi NDK was phylogenetically distant from protozoal NDK and devoid of the histone H3 domain, which was found in eukaryotic NDK. Two isoforms of CTP synthase were retrieved from camel genome with medium homology per cent. These are replaced by one isoform in T. evansi. In terms of drug targets, both NDK and CTP synthase showed conserved and phylogenetically and motif distinctions. This enrolls the two targets as a choice for drug development against T. evansi.
Key words: Camel, CTP synthase, genome, NDK nucleotide, Trypanosoma evansi, UTP
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