Z.I. Chaudhry, Jahanzaib, A. Aslam and I. Ahmed
Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan
ABSTRACT
Prevalence of Trypanosoma evansi infection among camels in Punjab (Pakistan) was studied using haematocrit centrifugation technique (HCT) and polymerase chain reaction (PCR). Two different primer pairs (one specific for T. evansi and the other for T. brucei) were used. Among 100 camels of either sex and all ages from different localities of Punjab, the prevalence was 4% by haematocrit centrifugation technique, 9% with PCR primer-1 and 13% with primer-2. The observations establishes evidence of the usefulness of PCR as a diagnostic tool for epidemiological studies.
Key words: Camel, PCR, prevalence, Trypanosoma evansi
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