Sharma S.K.1, Meena D.S.2, Yadav R.3, Meena D.4 and Nathawat P.3
1Department of Veterinary Microbiology and Biotechnology, 2Department of Veterinary Medicine,
Post- Graduate Institute of Veterinary Education and Research (PGIVER), Jaipur
3Department of Veterinary Microbiology and Biotechnology, College of Veterinary and
Animal Science, Bikaner, Rajasthan University of Veterinary and Animal sciences, Bikaner-334001 (Rajasthan), India.
4Department of Animal Husbandry, Government of Rajasthan, India
ABSTRACT
The present study was attempted to detect multidrug resistance pattern of Escherichia coli isolates obtained from nasal discharge of healthy and diseased camels. Out of total 112 samples of deep nasal swab (47 healthy and 65 diseased camels), 55 (20 from healthy and 35 from diseased camels) E. coli isolates were obtained. Further all isolates were subjected to 24 antibiotics of various groups and found marked difference in multidrug resistance profile of the isolates. All the 35 isolates from diseased camels were 100% sensitive to faropenem, gentamicin, imepenem whereas the all 20 isolates from healthy camels were 100% sensitive to ampicillin+sulbactam, cefepime, cephotaxime, ceftazidime, ciprofloxacin, faropenem, gentamicin, imepenem, norfloxacin and trimethoprim. Isolates from diseased camels showed 100% resistance to ampicillin, bacitracin, clindamycin and sulfadiazine whereas isolates from healthy camels showed 100% resistance to clindamycin and sulfadiazine. Overall resistance pattern revealed that all the 55 E. coli isolates were susceptible to faropenem, gentamicin and imepenem while resistant to clindamycin and sulfadiazine. Among isolates from healthy camels, 14 different resistotypes patterns were detected with 0.9579 discriminatory index and 0.13 to 0.38 multiple antibiotic resistance (MAR) index while isolates from diseased camels existed with 30 different resistotypes patterns, 0.9899 discriminatory index and 0.21-0.75 MAR index. The higher number of resistotypes, more discriminatory index and high value of MAR index indicated higher diversity and severity of MDR existed among E. coli isolates obtained from diseased camels.
Key words: Camel, Escherichia coli, MAR index, resistotype
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