K. Tyagi1, L. Gautam2, H.A. Waiz2, S.C. Mehta3 and B.K. Beniwal4
Department of Animal Genetics and Breeding, 4College of Veterinary and Animal Science, 2College of Veterinary and
Animal Science, Navania, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, Rajasthan
1Animal Husbandry Department, Government of Rajasthan
3ICAR-National Research Centre on Camel, Bikaner, Rajasthan, India
ABSTRACT
Eleven New World Camelids microsatellite primer pairs were used to investigate the genetic polymorphism in Bikaneri camel. Genomic DNA was extracted from blood and polymerase chain reactions were carried out for 30 unrelated camels of Bikaneri breed. Microsatellite technique was used for analysis of DNA. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Six microsatellite primer pairs could amplify the polymorphic microsatellite loci in Bikaneri camel. The number of alleles ranged from 2 to 5. The expected heterozygosity ranged from 0.289 to 0.686 and the polymorphic information content ranged from 0.267 to 0.639. The results indicated the utility of these microsatellite loci for studying genetic polymorphism in Indian dromedary and the potential use of microsatellite markers for further genetic investigations including individual identification, parentage testing and production enhancement.
Key words: Bikaneri, camel, genetic diversity, microsatellite markers, polymorphism
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