The objective of the present work was to purify bioactive components from camel milk and test them for antineoplastic properties. Camel milk was separated into casein and whey fractions and their cytotoxicity was assessed. Only camel milk whey and not casein were found to be cytotoxic to Hela cells. Further, camel milk whey was subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE), liquid chromatography-mass spectrometric (LC-MS) analysis and subsequent size exclusion chromatography. The cytotoxic potential of fractions obtained from chromatography were tested against the cervical cancer cell line, Hela. The present study indicated the presence of Lactoferrin (~77kDa) in the cytotoxic fractions purified from camel milk whey. This study indicates that camel milk whey and its components can be used as a nutraceutical with health benefis. It also suggests the potential of using camel milk lactoferrin as an important molecule with cyotoxic potential.
Key words: Camel milk, cervical cancer, chromatography, cytotoxicity, Hela, lactoferrin, LCMS, whey
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