Curriculum Vitae - Nisar Ahmad Wani (Member)

• Doctor of Philosophy (Ph. D.) from GB Pant University of Agriculture and Technology, India (1999 to 2003).
• Master of Veterinary Sciences (1993 to 1996) from SK university of Agricultural Sciences and Technology, Srinagar, India,
• Bachelor of Veterinary Sciences and Animal Husbandry (B. V. Sc. & A. H.), from SK university of Agricultural Sciences and Technology, Srinagar, India (1986 to 1992).

October 2014 – till date    Scientific Director, Reproductive Biotechnology Center, Dubai, UAE.   www.reprobiotech.ae

March 2005 – October 2014:  Head of Reproductive Biology Laboratory, Camel Reproduction Centre, Dubai, UAE.

April 2003 – March 2005:       Sr. Scientist, Central Veterinary Research Laboratory, Dubai, UAE.

Feb 1998 – April 2003:           Assistant Professor Cum Junior Scientist, SK University of Agricultural Sciences and Technology, Srinagar, India.

Feb 1992 – Feb 1998:            Senior Technical Assistant, SK University of Agricultural Sciences and Technology, Srinagar, India

• Produced World’s First Cloned Camelid, a dromedary camel named “Injaz”, from ovarian cells of a slaughtered animal.  https://doi.org/10.1095/biolreprod.109.081083
• Produced World’s First Cloned Bactrian Camel by interspecies Somatic Cell Nuclear Transfer (iSCNT), using dromedary camel as the source of ooplasm as well as surrogate to carry the pregnancy to term. https://doi.org/10.1371/journal.pone.0177800
• First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region. https://doi.org/10.3390/antibiotics13030227
• First report of embryo production by intra-cytoplasmic sperm injection (ICSI) in camels. DOI: 10.1016/j.theriogenology.2018.02.004
• First report of applying and optimizing the technique of ultrasound guided transvaginal ovum pick-up (OPU) in camels. DOI: 10.1016/j.theriogenology.2010.02.026
• First report of developing a pre-maturation system for cumulus oocyte complexes in camels using roscovitine.  DOI: 10.1016/j.theriogenology.2020.07.025
• First report of Chemical activation of in vitro matured dromedary camel (Camelus dromedarius) oocytes with optimization of protocols.  DOI: 10.1016/j.theriogenology.2007.11.011
• First report to study the kinetics of in vivo oocyte maturation in camels. DOI: 10.1016/j.theriogenology.2010.02.026
• First report of successful liquid storage of epididymal spermatozoa collected from slaughtered camels up to 8 days and production of embryos by IVF from such semen. DOI: https://doi.org/10.1016/j.anireprosci.2008.02.005
• First report on storage of ovaries and kinetics of oocyte maturation in camels. DOI: https://doi.org/10.1016/j.theriogenology.2004.11.009
• First report of embryo (blastocyst) production from cryopreserved immature oocytes in buffalo. DOI: 10.1016/j.theriogenology.2003.06.002
• First report of successful cryopreservation of ovarian tissue in buffalo. Ph D Thesis. G.B. Pant University of Agriculture and Technology, Pantnagar, India).

• Ghazawi, A.; Strepis, N. Anes, F.; Yaaqeib, D.; Ahmed, A.; AlHosani, A.; AlShehhi, M.; Manzoor, A.; Habib, I.; Wani, N.A.; et al. (2024). First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region. Antibiotics, 13, 227. https://doi.org/10.3390/antibiotics13030227
• H. Abouhefnaway and Wani NA(2023) Use of lactating females with nursing calves as recipients in an embryo transfer program in camel (Camelus dromedarius). Reproduction, Fertility and Development 36(2) 212. https://doi.org/10.1071/RDv36n2Ab119
• C. Liu, P. Kumar and Nisar Wani (2023).  Growth kinetics and single cell cloning capability of skin cell fibroblasts in dromedary camel (Camelus dromedarius). Reproduction, Fertility and Development 36(2) 159 https://doi.org/10.1071/RDv36n2Ab20
• SeungBum Hong and Nisar Ahmad Wani (2023). STUDIES ON SOME BIOCHEMICAL CONSTITUENTS OF FOLLICULAR AND OVIDUCTAL FLUID IN DROMEDARY CAMEL (Camelus dromedarius). Journal of Camel Practice and Research Vol 30 No 1, p 105-112.  DOI:10.5958/2277-8934.2023.00014.0
• WaniNA, Kumar KP, Hong SB, Umer MA (2022). Telomere length in dromedary camels (Camelus dromedarius) produced by somatic cell nuclear transfer (SCNT) and their age-matched naturally produced counterparts. Theriogenology,177:151-156. https://doi.org/10.1016/j.theriogenology.2021.10.012
• Vettical BS, WaniNA(2021). In vivo versus in vitro embryo production after super stimulation of donors in the camel (Camelus dromedarius). Reproduction, Fertility and Development 34(2) 269-270. https://doi.org/10.1071/RDv34n2Ab67
• Abouhefnawy HA, Wani NA (2021).  Storage of in vivo-produced embryos at refrigeration temperature before transfer to synchronized recipients in the camel (Camelus dromedarius). Reproduction, Fertility and Development 34(2) 277-277. https://doi.org/10.1071/RDv34n2Ab81
• Wani NA, Kumar KP (2021). Telomere length in cloned camels produced by somatic cell nuclear transfer is not different from that of their naturally produced counterparts. Reproduction, Fertility and Development 33, 114-114. https://doi.org/10.1071/RDv33n2Ab14
• Abouhefnawy H. A., Wani N. A. (2021).  Use of embryo transfer technology to salvage the germplasm of elite dromedary camels (Camelus dromedarius) infected with brucellosis. Reproduction, Fertility and Development 33, 144-144. https://doi.org/10.1071/RDv33n2Ab73
• Vettical B. S., Wani N. A. (2021). Body condition of donor animal and recipients affects the outcome of embryo transfer program in dromedary camel (Camelus dromedarius). Reproduction, Fertility and Development 33, 144-145. https://doi.org/10.1071/RDv33n2Ab74
• Abouhefnawy H. A., Wani N. A. (2021). Effect of season on the super stimulation response, embryo quality, and pregnancy establishment in dromedary camel (Camelus dromedarius). Reproduction, Fertility and Development 33, 146-146. https://doi.org/10.1071/RDv33n2Ab76
• Wani, NA (2021). In vitro embryo production (IVEP) in camelids: Present status and future perspectives. Reproductive Biology, Volume 21, Issue 1, 2021, 100471. https://doi.org/10.1016/j.repbio.2020.100471.
• Hong SB, Vettical B, Wani NA (2020). Development of prepubertal goat oocytes after their in vitro maturation and chemical activation. Zygote 28, 447-452.  Doi: 10.1017/S0967199420000313
• Wani NA, Hong SB (2020). Effect of roscovitine pretreatment on in vitro maturation of oocytes and their subsequent developmental after chemical activation in dromedary camel (Camelus dromedarius). Theriogenology, 157, 176-180.  https://doi.org/10.1016/j.theriogenology.2020.07.025.
• Skidmore JA, Crichton EG, Malo C, Vaughan JL, Wani NA, Herrid M.  Reproductive technologies in camelids. In book: Reproductive Technologies in Animals 2020 DOI: 10.1016/B978-0-12-817107-3.00008-4
• Vettical B, Hong SB, Umer, MA and Wani NA (2019). Comparison of pregnancy rates with transfer of in vivo produced embryos derived using multiple ovulation and embryo transfer (MOET) with in vitro produced embryos by somatic cell nuclear transfer (SCNT) in the dromedary camel (Camelus dromedarius). Animal Reproduction Science 209:106132. DOI: 10.1016/j.anireprosci.2019.106132
• Wani NA and Hong SB. In vitro maturation of pre-pubertal goat oocytes and their development after chemical activation. Reproduction Fertility and Development 2019 31(1): 205
• Wani NA, Hong S, Vettical BS (2018). Cytoplast source influences development of somatic cell nuclear transfer (SCNT) embryos in vitro but not their development to term after transfer to synchronized recipients in dromedary camels (Camelus dromedarius). Theriogenology 118:137-143. https://doi.org/10.1016/j.theriogenology.2018.05.021
• Wani NA, Hong SB (2018). Intracytoplasmic sperm injection (ICSI) of in vitro matured oocytes with stored epididymal spermatozoa in camel (Camelus dromedarius): Effect of exogenous activation on in vitro embryo development. Theriogenology 8; 113: 44-49.  https://doi.org/10.1016/j.theriogenology.2018.02.004
• Wani NA, Hong SB (2018). Source, treatment and type of nuclear donor cells influences in vitro, and in vivo development of embryos cloned by Somatic Cell Nuclear Transfer in camel (Camelus dromedarius). Theriogenology 15; 106: 186-191. https://doi.org/10.1016/j.theriogenology.2017.10.010
• Wani NA, Vettical BS, Hong SB (2017). First cloned Bactrian camel (Camelus Bactrians) calf produced by interspecies somatic cell nuclear transfer: A step towards preserving the critically endangered wild Bactrian camels. PLOS ONE 12(5): e0177800. https://doi.org/10.1371/journal.pone.0177800
• Wani NA, Binoy VS, and Hong SB (2017). Recent Advances in Cloning by Somatic Cell Nuclear Transfer in Camelids. Reproduction, Fertility and Development 30(1) 156-156
• Binoy S Vettical, S B Hong, Nisar A Wani (2016). Multiple ovulation and embryo transfer (MOET) in camels: An overview. Asian Pacific Journal of Reproduction 5; 2:102-104. https://doi.org/10.1016/j.apjr.2016.01.003
• Wani NA (2012).Status of cloning by somatic cell nuclear transfer (SCNT) in camels” Proceedings of 3^rd conference of International Society of Camelid Research and Development (ISOCARD) Pp 28-29.
• Wani NA, KM Morton, M Billah, Skidmore JA (2011). Biophysical and biochemical characteristics of ejaculated dromedary camel (Camelus dromedarius) and llamas (Llama glama) semen. J Camel Practice and Research 18, 97-102.
• Wani NA, Wernery U, Hassan FAH, Wernery R, Skidmore JA (2010). Production of the First Cloned Camel by Somatic Cell Nuclear Transfer. Biology of Reproduction82, 373–379. https://doi.org/10.1095/biolreprod.109.081083
• Wani, NA, Wernery U (2010). Effect of different protein supplementations and epidermal growth factor on in vitro maturation of dromedary camel (Camelus dromedarius) oocytes. Reproduction in domestic animals45, e189–e193
• Wani NA, Skidmore JA (2010).Ultrasonographic-guided retrieval of in vivo matured oocytes after super-stimulation in dromedary camel (Camelus dromedarius). Theriogenology74; 436–442. https://doi.org/10.1016/j.theriogenology.2010.02.026
• Kinne J, Wani N A,Wernery U, Peters J and Knospe C (2010). Is There a Two-Humped Stage in the Embryonic Development of the Dromedary? Anatomia, Histologia, Embryologia. Doi: 10.1111/j.1439-0264.2010. 01011.
• Wani NA (2009). In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4⁰C. Animal Reprod Sci. 111; 69–79. https://doi.org/10.1016/j.anireprosci.2008.02.005
• Wani NA, Skidmore JA and Wernery U (2009).Preliminary studies on the development of reconstructed embryos after nuclear transfer in dromedary camel (Camelus dromedarius). ReprodFertil Dev 21; 128-129.
• Wani NA, Billa M, Skidmore JA (2008). Studies on liquefaction and storage of ejaculated dromedary camel (Camelus dromedarius) semen. Animal Reprod Sci.  109; 309–318. https://doi.org/10.1016/j.anireprosci.2007.10.011
• Wani, NA (2008).In vitro embryo production in camelids: An overview. Proceedings WBC/ ICAR 2008 Satellite Meeting on Camelid Reproduction, Budapest, Hungary, Pp 46-50.
• Wani NA (2008). Chemical activation of in vitro matured dromedary camel (Camelus dromedarius) oocytes: optimization of protocols. Theriogenology 69; 5: 591-602. https://doi.org/10.1016/j.theriogenology.2007.11.011
• Wani, NAand Skidmore JA (2008). In Vitro maturation of dromedary camel (Camelus dromedarius) oocytes: Effect of different protein supplements and epidermal growth factor. Reproduction in Domestic Animals 43: 96.
• Wani, NA (2008). Development of Camel (Camelus dromedarius) oocytes after chemical activation.ReprodFertil Dev 20; 1: 197.
• Wani NA and Wernery U (2007).  Production of dromedary embryos from gametes obtained from slaughterhouse. Proceedings of International Camel Conference on “Recent Trends in Camelid Research and Future Strategies for Saving Camels”, Feb 16-17, 2007, Bikaneer, India
• Wani NA and Wernery U (2006). Parthenogenetic activation of camel (Camelus dromedarius) oocytes. Biology of Reproduction (special issue), 180.
• Wani NA and Wernery U (2006). Effect of different protein supplementations on the in vitro maturation of camel (Camelus dromedarius) oocytes. Proceedings, International Scientific Conference on Camels, May 9- 11, 2006, Gassim University, Buraida, Kingdome of Saudi Arabia.
• Wani NA and Nowshari MA (2005). Kinetics of nuclear maturation and effect of holding ovaries at room temperature on in vitro maturation of camel (Camelus dromedarius) oocytes. Theriogenology 64:75-85.
• Wani NA and Nowshari MA (2005).  Effect of heparin and calcium ionophore on acrosome reaction in epididymal spermatozoa of dromedary camel (Camelus dromedarius). ReprodFertil Dev 17: 250-251.
• Nowshari MA and Wani NA (2005). Camelid embryo development in vitro: effect of protein supplementation in maturation medium and subsequent culture in two different media on fertilization and development.  ReprodFertil Dev 17: 276.
• Wani NA, Nowshari MA and Wernery U (2005) Storage of camel (Camelus dromedarius) epididymal spermatozoa in different extenders and at different temperatures. Biology of Reproduction (special issue), 234