Aminu Deen, Sumant Vyas, Mamta Jain and M.S. Sahani
National Research Centre on Camel, P.B.No. 07, Jorbeer, Bikaner, INDIA
ABSTRACT
A total of 28 semen samples were collected from 6 males, each ejaculate was split into two and extended with Tris and Biociphos dilutors, respectively. The semen samples were extended at room temperature and were slowly cooled to 4°C. The individual sperm motility was recorded at 0, 24, 48, 72, 96 and 120 hours or till the motility ceased. None of the 28 samples extended in Biociphos could retain motility at 24 hours after collection while 11/28 (39.28%), 10/28 (35.71%), 5/28 (17.85%) and 3/28 (10.71%) semen samples extended in Tris could retain motility for 24, 48, 72 and 96 hours, respectively. The study was then continued with Tris buffer and 66 more semen samples (totaling 94) were extended and evaluated for preservability of sperm motility. The results indicated that 76/94 (80.85%), 35/94 (37.23%), 25/94 (26.59%), 9/94 (9.57%), 5/94 (5.32%) and 1/94 (1.06%) samples extended in Tris could retain motility for 0, 24, 48, 72, 96 and 120 hours, respectively. It is concluded that Biociphos is not a suitable extender for preservation of camel semen and preservability is also low in Tris extender. The composition of Tris buffer need to be modified for improving preservability of camel semen.
Key words : Camel, semen preservation, Tris and Biociphos extenders
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