Camels and Camelids

PREVALENCE OF ROTAVIRUS INFECTION IN CAMELS AND OTHER ANIMAL SPECIES

Journal Edition: April 2021
Article DOI: 10.5958/2277-8934.2021.00016.3
Published On: 04-04-2021 05:46

Abdelrahman Taha Hereba1, Mohammed Soliman Shathele1 and Maged Gomaa Hemida1,2
1Department of Microbiology, College of Veterinary Medicine, King Faisal University, Saudi Arabia
2Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt


ABSTRACT


Rotaviruses are among the main causes of enteritis in naive humans, animals, and birds. The affected animals or birds showing signs of enteritis in the form of diarrhoea, emaciation, dehydration and finally death in some cases. Little is known about its prevalence and the circulating strains of the virus is not well characterised in the eastern province of Saudi Arabia. The main objective of the current work was to detect and study the morphology of rotavirus particles in faecal suspensions from various animals from the eastern province of Saudi Arabia. To achieve these goals, we collected 140 faecal samples from dromedary camels, chicken, sheep, goat, and turkeys showing signs of diarrhoea. We processed these faecal specimen and prepared tissue suspension per each sample. We tested these faecal suspensions with the commercial available Rotavirus latex agglutination kits. Our results showed 5.17% of sheep samples positive. This represented about 2.14% of the overall tested samples from all species. We further tested these positive samples by the transmission electron microscope technique (TEM). The TEM pictures showed a typical Rotavirus shape in icosahedral in symmetry, and wheel shape. The morphometric analysis of the virus particles revealed the size of the virus ranging from 60-75 nm in diameter. These results suggested the potential roles of sheep in the transmission of rotavirus to other species of animals particularly dromedary camels living in their close proximity. There was a consistency in the results of both the latex agglutination tests and the electron microscope in the detection of rotavirus infection in faecal samples of different animals species studied.
Key words: Diarrhoea, dromedary camels, EM, enteritis, latex agglutination, rotavirus, sheep