Camels and Camelids

LOCALISATION OF ESTRASE IN THE BLOOD CELLS OF CAMEL AND BUFFALO AT ULTRASTRUCTURAL LEVEL – PART I

Journal Edition: June 2012
Article DOI:
Published On: 03-09-2018 19:11

P.K. Yadav1 and Gurdial Singh2
1Animal House Facility, AIIMS, New Delhi
2Department of Veterinary Anatomy, College of Veterinary Sciences,
Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar 125 004, India

ABSTRACT

The present study was conducted to localise ultrastructurally different enzymes in the leucocytes of buffalo and camel. The blood from each animal was collected from the jugular vein in siliconised test tubes with EDTA (Ethylene diamine tetra acetate) as an anticoagulant. The blood samples were then processed and stained for Non–Specific Esterase, Arylsulfatase C & Arylsulfatase B. The non-specific esterase activity using alpha-naphthyl acetate restricted mainly in the lymphocytes in the form of large globules in camel and buffalo blood while the activity with reference to alpha-naphthyl butyrate in addition to lymphocytes was also seen in few neutrophils and eosinophils of camel. Arylsulfatase B and Arylsulfatase C reactivity was more in eosinophils of camel as compared to neutrophils. Within the neutrophils the reactivity of arylsulfatase C was seen within the granules but for arylsulfatase B it was distributed within the cytoplasm and over the nuclear membrane. Few basophils of buffalo also exhibited positive reactivity for arylsulfatase B and arylsulfatase C.
Key words: Arylsulfatase B, Arylsulfatase C, buffalo and camel, leucocytes, non–specific esterase, transmission electron microscopy