Homayon Reza Shahbazkia1, Amin Bigham Sadegh2, Mohammad Shadkhast4, Afshin Jafari Dehkordi3 and Mohammad Hossein Mahdavi1
1Department of Biochemistry, 2Department of Veterinary Surgery and Radiology, 3Department of Large Animal Medicine, 4Department of Anatomy and Histology, Faculty of Veterinary Medicine,
Shahrekord University, Shahrekord 115, Iran
ABSTRACT
Glycated haemoglobin (HbG) concentration is a retrospective measure of mean blood glucose level and is not affected by recent stresses, food ingestion or exercise. HbG has been determined in various wild and domestic animals such as kestrels, markhor, mouflon, aoudad, deer, goat, sheep, dog, camel and horse. But there is no information about HbG in Iranian cross-bred camels. On the other hand, relation of HbG to blood glucose and its value in metabolic profiling has not been clarified in the camel. The purpose of this study were to determine normal value of glycated haemoglobin in adult Iranian cross-bred camels and to investigate its relation to fasting plasma glucose. Blood samples were collected from jugular veins of 45 clinically healthy adult camels (20 males and 25 females). After separation and washing of red blood cells, hemolysate was prepared and subjected to weak cation exchange chromatography for determination of HbG. Glucose was measured in plasma samples. Plasma glucose and HbG in the camels were 109.7 ± 13.24 mg/dl and 4.12 ± 0.96% of total haemoglobin. It was shown that HbG% and plasma glucose correlated together (r = 0.78, p<0.05).
Key words: Camel, cation exchange chromatography, glycated haemoglobin (HbG)
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