Camels and Camelids

DANOFLOXACIN AND MARBOFLOXACIN ACTIVITY ON SOME BACTERIAL ISOLATES FROM CAMELS (Camelus dromedarius)

Journal Edition: December 2015
Article DOI: 10.5958/2277-8934.2015.00032.6
Published On: 03-09-2018 19:06

M. Ait Lachguer1, M. Boulahna3, N. Ziyate1, A. Soulaymani2, M. Oukessou3 and A. El Hraiki3
1Office National de Sécurité Sanitaire des Aliments, Division de Pharmacie et des Intrants
Vétérinaires, Cité Yakoub El Mansour, Rabat, Morocco
2Laboratoire de Génétique et Biométrie, Faculté des Sciences, Université Ibn Tofail, Kénitra, Morocco
3Département des Sciences Biologiques et Pharmaceutiques Vétérinaires- Institut
Agronomique et Vétérinaire Hassan II, Rabat, Morocco

ABSTRACT

Bacterial diseases in camel cause morbidity, mortality, suffering and significant economic losses. This study assessed the types of bacteria involved in different types of lesions encountered in camel’s lung and liver slaughtered at municipal slaughter houses of Rabat, Morocco. Bacterial species isolated and identified included Staphylococcus aureus (19.2%), Staphylococcus sp (46.1%), Streptococcus sp (7.6%), Corynebacterium sp (15.3%) and Acinetobacter sp (11.5%). The percentage of resistance of isolates to the antibiotics varied from 0 to 84.6%. The most frequent resistance was to Ampicillin and Penicillin G (84.6%) followed by Gentamycin and Tetracycline (11.5. %), while all the isolates were susceptible to Cephalothin and Ciprofloxacin. Minimum inhibitory concentrations (MIC) of danofloxacin and marbofloxacin were determined against these bacteria. Time-kill curves against staphylococcus aureus (MIC=0.25μg/ml), Streptococcus sp (MIC=0.5μg/ml), Corynebacterium sp (MIC=0.5μg/ml) for both danofloxacin and marbofloxacin, and Acinetobacter sp (MIC=0.125μg/ml for marbofloxacin and 0.25 μg/ml for danofloxacin) were then determined according to a broth microdilution test. The pharmacodynamic parameters as lowest effective concentration (LEC) and optimal bactericidal concentration (OBC) were determined. Optimal values of surrogate markers predicting the antimicrobial effect and preventing the development of resistance were widely reached.
Key words: Camel, danofloxacin, marbofloxacin, MIC, time-kill curves