CHLORTETRACYCLINE IN SERUM AND MILK FOLLOWING SINGLE INTRAUTERINE ADMINISTRATION IN CLINICAL ENDOMETRITIS CAMELS (Camelus dromedarius)

I.M. Ghoneim1,2, A.M. Abdelghany1,3, M.M. Waheed1,2, M. Bazalou4,5,M.M. Al-Eknah1 and K.Al-Busadah6
1Department of Clinical Studies, 6Department of Physiology, Biochemistry and Pharmacology College of Veterinary Medicine, King Faisal University, Saudi Arabia, Al-Ahsa 31982, Al-Hufof P.O. 400
2Department of Theriogenology, College of Veterinary Medicine, Cairo University, Giza 12211
3Department of Physiology, 5Animal Health Research Institute (AHRI), Agricultural Research Centre, Giza, Egypt
4Toxicology, Drugs and Hormones Residues Lab., Ministry of Agriculture, Riyadh, Kingdom of Saudi Arabia

ABSTRACT

This research paper aimed to study the effect of single intrauterine administration of Chlortetracycline (CTC) in clinical endometritis camels (Camelus dromedarius) with regard to its levels in serum and milk. CTC pessaries were administered to 5 dromedary camels with clinical endometritis at a dosage of 2 g per animal. Blood and milk samples were collected before treatment and at 12 h intervals for 156 h. Serum and milk were analysed by ultra-performance liquid chromatography (UHPLC/MSMS). The serum and milk analyses revealed that maximum concentration of CTC was detected at 12 h post-treatment. The mean maximum CTC concentration was recorded in the serum and milk at 12 h post CTC administration. The maximum individual concentrations of CTC in milk ranged from 434.0 to 34.6 ng/ml. The mean concentration of CTC in the serum and milk decreased steadily by 24 and 36 h and thereafter post-treatment, respectively. CTC sustained in the milk during the period of 144.4 ± 13.99 h (range, 118 to 154 h) where the serum CTC retained by 111.2 ± 11.70 h (range, 106 to 130 h) after treatment. The mean milk CTC values ≥30 ng/ml was proved till 24 h after treatment (range 12 to 60 h). In conclusion, the safe residual level (≥30 ng/ml milk) established by the US Food and Drug Administration was recorded in the dromedary milk at 24 h onward after intrauterine CTC administration.
Key words: Camel, chlortetracycline, endometritis, milk, uterus