Layla I. Mohamed1 and M.M. Mukhtar2
1Department of Bacterial Vaccines Production, Central Veterinary Research Laboratories,
P.O. Box 8067 Khartoum, Sudan
2Department of Molecular Biology, Institute of Endemic Diseases P.O. Box 11463, University of Khartoum, Sudan
ABSTRACT
A colorimetric assay using MTT salt was used to measure the in vitro proliferative response of peripheral blood monocytes (PBMs) of Sudanese camels vaccinated with Brucella abortus strain 19 vaccine. The mean stimulation index of PBMs of camels after vaccination was significantly higher than before vaccination (P < 0.01). The results suggest possible protection of camels from brucellosis using strain 19 Brucella abortus vaccine.
Key words: Brucella abortus strain 19 vaccine, camels, cellular immunity, peripheral blood monocytes