Journal Year: 2013
Journal Month: June
Published On: 03-09-2018 19:09:00
Article DOI:

H. Arabha Najafabadi1, A. Niasari-Naslaji1 , A.B. Atakpour1, S. Ghaffari1, A. Barin1, H.R. Samiei2,M. Amjadi3, A.A. Haji Moradlou2, A. Salimi4, I. Narimani4 and A. Gerami5
1Department of Clinical Science, Faculty of Veterinary Medicine
5School of Mathematics, Statistics and Computer Science, University of Tehran, Tehran, Iran
2Pegah Milk Company, Golestan, Iran
3International Dairy Federation, Tehran, Iran. 4Centre for Animal Breeding, Ministry of Jahad-e-Agriculture, Iran


Effect of camel preservation milk on SCC and bacteriological isolation was investigated. Samples were collected from individual quarters (n=16) of 5 milking camels. Five minutes after receiving oxytocin (20 I.U), teat was washed and calf was released to stimulate the dam. Next, the suckling was interrupted, the teat was dried with tissue and the sample was collected aseptically for bacterial culture. Subsequently, milk samples were collected for SCC into tube with and without potassium dichromate. Frozen samples were thawed at room temperature and cultured one week after collection. For 4 days after milk samplings, SCC and culture of milk was conducted on a daily basis, maintaining the sample at 4°C. Out of 16 samples, 6 cases were free from any bacterial contamination and in 10 cases single pure bacteria were isolated. The possibility of isolating bacteria from fresh sample on the day of milk collection was greater than frozen specimen (p=0.07). SCC in milk samples without preservative decreased steadily during 4 days storage (P<0.0001); however, such decrease was not noticed in samples with preservative (P>0.05). Freezing adversely affected SCC with or without preservative. In conclusion, preservation of camel milk samples with potassium dichromate over 4 days at 4°C is a valid method to investigate SCC. However, due to the inconsistency in bacteriological isolation following storage at 4°C and freezing, it is advised to perform bacteriological investigation immediately following milk sampling in camel.
Key words: Bacterial isolation, camel milk, preservation, somatic cell count

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