MOLECULAR CHARACTERISATION OF KACHCHHI CAMEL (Camelus dromedarius) USING MICROSATELLITE MARKERS
Journal Year: 2012
Journal Month: December
Published On: 03-09-2018 19:11:00
Article DOI:

R.C. Parikh, N.A. Patel, Y.D. Padheriya1, K.N. Wadhwani1 and D.N. Rank
Department of Animal Genetics and Breeding, 1Department of Livestock Production and Management,
College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand - 388 001, Gujarat, India

ABSTRACT

Molecular characterisation of Kachchhi camel was carried out using 16 microsatellite markers. A total of 74 blood samples were collected randomly from non-related animals and DNA was extracted. Sixteen microsatellite loci were amplified in a 5 multiplex PCR. Out of 16 microsatellite loci 14 were found to be polymorphic whereas, 2 were found monomorphic. The estimated mean allelic diversity was 3.1 (± 1.68), with a total of 51 alleles. The genetic variability within this breed was observed in terms of mean observed 3.18 ± 1.68 and effective number of alleles (2.0 ± 0.98), observed heterozygosity (0.36 ± 0.21), expected Nei’s heterozygosity (0.421 ± 0.22). The PIC values ranged from 0.206 to 0.711. The Shanon’s index ranged from 0.3960 to 1.5087 with mean 0.733 ± 0.450. These data were further used to verify if this population had undergone genetic bottleneck in the recent past. The result indicated existence of enough genetic variation and no bottleneck in this population.
Key words: Kachchhi camel, microsatellite markers, molecular characterisation


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