IN VITRO DIFFERENTIATION OF ENDOTHELIAL PROGENITOR STEM CELLS DERIVED FROM PERIPHERAL BLOOD OF CAMEL
Journal Year: 2012
Journal Month: December
Published On: 03-09-2018 19:11:00
Article DOI:

Hala M.F. El Miniawy1, Dina Sabry2 and Laila A. Rashed2
1Pathology Department, Faculty of Veterinary Medicine, Cairo University, Egypt
2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt
CCS: Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

ABSTRACT

The peripheral blood-derived mononuclear cells (PBMNCS) obtained from camel blood when set in culture gave rise to adherent cells. The count of colony forming unit (CFU) after culturing PBMNCS for 7 days were significantly lower in the non pregnant animals than in the pregnant ones (32.4 ± 26.7 versus 65.2 ± 18.3, respectively). Flow cytometry analysis shows the expression of CD34 + (33.8%), CD14 + (33%) and CD29 + (22.8%), the latter is a marker for mesenchymal stem cells although direct evidence for the presence of mesenchymal progenitor cells (MPCS) in the peripheral blood does not exist. The stem/ progenitor cell characteristics were evidenced by their proliferative capacities and their ability to differentiate into osteoblasts, chondrocytes and neural cells after culture for 2 months in a modified conditioned medium. The stemness capacity of the PB-MNCS of camel was proven in this study and further study was needed to establish the clinical application of these stem cells.
Key words: Camel, differentiation, endothelial progenitor cells, peripheral blood, stem cells


Price 50

       Add to cart