CORRELATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE AGOUTI SIGNALING PROTEIN (ASIP) AND A TYPE III RECEPTOR PROTEIN-TYROSINE KINASE (KIT) LOCI WITH COLOUR IN THE BACTRIAN CAMEL (Camelus bactrianus)
Journal Year: 2018
Journal Month: August
Published On: 15-10-2018 08:23:00
Article DOI: 10.5958/2277-8934.2018.00020.6

Liang Ming1, Dalai Siren2, Tuya Saren1, Li Yi1, Jing He1, Le Hai1, Fucheng Guo1 and Rimutu Ji1,2
1Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and
Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China
2Camel Research Institute of Inner Mongolia, Alashan, Inner Mongolia, 737300, China

ABSTRACT

Coat colour is an important characteristic and economic trait in bactrian camels. The agouti signaling protein (ASIP) and a type III receptor protein-tyrosine kinase (KIT) that control melanogenesis are candidate coat colour genes in mammals. However, these genes are not well characterised in bactrian camels and their association with colour has not been established. Here, we sequenced the ASIP and KIT genes in a population of different coloured bactrian camels to gain an improved understanding of the effects of these genes on coat colour. Our analysis of the bactrian camel ASIP gene revealed one mutation that altered the amino acid sequence of the encoded protein. In the KIT gene, we detected nine mutations, of which six were silent mutations and three were single nucleotide polymorphisms (SNPs) that alter the amino acid sequence (V28A, H57R, T118A). Analysis of associations between phenotypic and genotypic characteristics revealed that none of the mutations in the ASIP and KIT loci correlated completely with coat colour in bactrian camels. Thus, our findings indicate that the coat colour phenotype of the bactrian camel is not related to the ASIP and KIT genes; however, further investigations using more advanced technology with larger numbers of animals are required to confirm this conclusion.
Key words: Agouti gene, bactrian camel, coat colour, KIT gene, polymorphism, SNP


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