CLONING AND BIOINFORMATICS REVEALED MOLECULAR CHARACTERISATION OF CYP2E1 GENE FROM BACTRIAN CAMEL (Camelus bactrianus)
Journal Year: 2020
Journal Month: April
Published On: 27-04-2020 18:14:00
Article DOI: 10.5958/2277-8934.2020.00014.4

Liang Ming1 , Dalai Siren1,2, Li Yi1 and Rimutu Ji1,2 1 Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China 2 Camel Research Institute of Inner Mongolia, Alashan, Inner Mongolia, 737300, China

ABSTRACT

In the present study, the CYP2E1 gene from Bactrian camel (Camelus bactrianus) was cloned, sequenced and explored for its structural and functional aspect. The full-length sequence of CYP2E1 gene was 1754bp long, contained a coding region of 1488bp open reading frame (ORF), which was predicted to encoding 495 amino acid residues. The comparison between CYP2E1 from Bactrian camel (Camelus bactrianus) and other animal species showed that nucleotide and encoded amino acid sequences of the Bactrian camel CYP2E1 gene exhibited high similarity with the wild Bactrian camel (Camelus ferus), and the phylogenetic analysis revealed that Bactrian camel (Camelus bactrianus) CYP2E1 was grouped with that of wild Bactrian camel (Camelus ferus). The CYP2E1 protein contained a transmembrane domain which extended from 2-23 amimo acids, while 24-495 amino acids were outside the cell, and no signal peptide was found. The prediction of tshe secondary structure revealed that there had 44.04% alpha helix, 7.07% beta fold, and 48.89% random coils. Our research may be valuable for exploring the function and detoxification molecular mechanisms underlying the molecule.

Key words: Bactrian camel, bioinformatics, CYP2E1 gene


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