Jamal Hussen1*, Abdullah I. A. Al-Mubarak1, Naser A. Al Humam1,Sameer M. Alhojaily2,3 and Ali Fadlelmula1
1Department of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Ahsa, Saudi Arabia
2Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa, Saudi Arabia
3Agricultural and Veterinary Training and Research Station, King Faisal University, Al-Ahsa, Saudi Arabia
The aim of the present study was to use staining with monoclonal antibodies to different cell surface molecules and flow cytometry to analyse the expression pattern of cell markers on camel blood eosinophils. Based on their light scatter charecterestics and green autofluorescence, camel eosinophilic granulocytes were identified as SSChigh/FSClow/Fl-1high cells within the granulocyte population. In comparision to neutrophilic granulocytes, camel eosinophils showed higher abundance of the cell surface molecules CD45, CD44, and CD11a but lower abundance of the cell markers CD172a and CD14. Collectively, the findings of the current study suggests a similar phenotype of camel, human, and bovine eosinophils.
Key words: Adhesion molecules, camel, eosinophilic granulocytes, flow cytometry, immunophenotype