Camels and Camelids

THE ACTIVITY OF GLYCOSIDASES (β -N-ACETYLGLUCOSAMINIDASE, α-N-ACETYLGALACTOSAMINIDASE AND α L FUCOSIDASE) IN THE UTERINE LUMINAL FLUID AND BLOOD SERUM OF THE DROMEDARY CAMEL (Camelus dromedarius) DURING THE FOLLICULAR CYCLE

Journal Edition: August 2021
Article DOI: 10.5958/2277-8934.2021.00022.9
Published On: 27-07-2021 09:51

I.M. Ghoneim1,2, M.M. Waheed1,2, A.M.A. Meligy1,3 and S.M. El-Bahr4,5
1Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa, 31982, Saudi Arabia
2Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12515, Egypt
3Animal health Research Institute, Agricultural Research Centre, Giza, Egypt
4Department of Biomedical Sciences (Biochemistry), College of Veterinary Medicine,King Faisal University, Al-Ahsa, 31982, Saudi Arabia
5Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Egypt
ABSTRACT


The activity of three glycosidases (β-N-acetylglucosaminidase, α-N-acetylgalactosaminidase and α-L-fucosidase) were estimated in the uterine fluid and serum throughout the follicular cycle of 24 slaughtered dromedary camels. These camels had ovaries bearing different size follicles (0.5-20mm) that grouped into group I (5–10 mm), group II (11–15 mm) and group III (16–20 mm). β-N-acetylglucosaminidase, α-N-acetylgalactosaminidase and α-L-fucosidase were determined in the uterine fluid and serum samples using ELISA kits. Results indicated that mean concentration of β-N-acetylglucosaminidase in uterine fluid was 3.33 ± 0.26, 2.73 ± 0.15, and 6.43 ± 1.41 ng/ml in Group I, II, and III, respectively. The level of α-N-acetylgalactosaminidase in uterine fluid was 3.21 ± 0.27, 2.22 ± 0.14, and 7.24 ± 1.45 ng/ml in Group I, II, and III, respectively. α-N-acetylgalactosaminidase concentration showed statistical differences (P<0.05) among the different groups. The value of α-L-fucosidase in uterine fluid was 435.00 ± 6.94, 340.00 ± 9.82, and 362.50 ± 31.92 μmol/ml in Group I, II, and III, respectively. The maximum mean activity of α-L-fucosidase (P<0.05) was reported in-group I. In group I, the concentrations of β-N-acetylglucosaminidase and α-N-acetylgalactosaminidase were significantly (P<0.05) higher in the uterine fluid than the blood serum. In-group II, the concentration of α-N-acetylgalactosaminidase in the serum was significantly higher (P<0.05) than in the uterine fluid. In-group III, β-N-acetylglucosaminidase, α-N-acetylgalactosaminidase and α-L-fucosidase concentrations were significantly (P<0.05) higher in uterine fluid than in the serum. The results proposed that β-N-acetylglucosaminidase, α-N-acetylgalactosaminidase and α-L-fucosidase might play a role in carbohydrate-mediated events in the uterus of dromedary camels.
Key words: Camel; Uterine fluids; α L fucosidase, α-N-acetylgalactosaminidase; β-N-acetylglucosaminidase