A. Niasari-Naslaji1, A.B. Atakpour1, B. Khoramian2, M. Emaneini3, S. Ghaffari1, H. Parsakiya1,A. Barin4, B.R. Samiei5, M. Amjadi6, V. Akbarinejad and A.A. Moosavi-Movahedi7
1Department of Theriogenology, 4Department of Clinical Pathology, Faculty of Veterinary Medicine,
7Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
3Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5Pegah Milk Company, Golestan, Iran
6International Dairy Federation, Tehran, Iran
ABSTRACT
Camel milk samples were randomly and aseptically collected after discarding first milk streak from 243 quarters of milking camels without clinical sign of mastitis or bacteriological investigation. There was no bacterial isolation in 61.73% samples. The bacteria isolated in remaining samples were Staphylococci (n=78; coagulase negative Staphylococci: 71 and Staph. aureus: 7) and Streptococci (n=26). Five new species of coagulase negative Staphylococci including Staph. saprophyticus, Staph. delphini, Staph. capitis, Staph. chromogenes and Staph. caseolyticus were identified. PFGE analysis carried out on 7 isolates of Staph. aureus and produced 2 distinct pulsotypes designated as pulsotypes A and B. All Staph. aureus isolates were found to be included into 2 spa types: t527 and t1532. Coagulase negative staphylococci were the main bacteria isolates (55.04%) and the frequency of Staph. aureus with 2 spa types was considered as 5.43% of total bacterial isolation.
Key words: Dairy dromedary camel, S. aureus, subclinical mastitis
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