Vijayata Choudhary1, Samar Kumar Ghorui2, Abhishek Gupta1, Sonu Yadav1 and Monika Choudhary1
1Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan, India
2NRCC, Jorbeer, Bikaner, Rajasthan, India
The purpose of this study was to determine clinical and subclinical prevalence of T. evansi among camels in Bikaner, India. In the present study, camels were examined parasitologically by blood smear examination and by Polymerase chain reaction (PCR) targeting ITS region of T. evansi. Blood samples of 74 camels were collected during the period from July 2019 to November 2019. The blood smear examination and molecular analysis showed 6.75 and 21.62%, respectively prevalence of T. evansi infection in camels, suggesting high sensitivity of diagnosis towards molecular tests. In comparison to parasitological methods, PCR proved fast, precise, sensitive detection diagnostic method for trypanosome infected camels. The adult camels possessed acute infection of 28.88% of T. evansi when compared to young camels with 10.34% infection, suggesting no significant difference (χ2=1.141, P > 0.05). Hence, molecular diagnosis targeting ITS region among the infected trypanosome camels is more reliable and accurate for epidemiological survey and control programmes of trypanosomosis caused by T. evansi.
Key words: Camel, diagnosis, polymerase chain reaction (PCR), Trypanosoma evansi, trypanosomiasis