Zhuwei Guo1, Xiangjun Xu2, Yongsheng Zhong3, Zhorigtu4, Chunhua Cao3, Xinji Jiang5, Demtu Er1* and Bin Yang1*
1College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs of P. R. China, Hohhot, 010018, China
2Alxa Left Banner Bayannorogon Comprehensive Administrative Law Enforcement Bureau, Bayannorogon, 750300, Inner Mongolia Autonomous Region, P.R. China
3Ejina Banner Centre of Animal Disease Prevention and Control, Dalaihub, 735400, Alxa League,
Inner Mongolia Autonomous Region, P.R. China
4Alxa League Institute of Animal Husbandry, Bayanhot, 750306, Inner Mongolia Autonomous Region, P.R. China
5Alxa Left Banner Centre of Animal Disease Prevention and Control, Bayanhot, 750300,
Alxa League, Inner Mongolia Autonomous Region, P.R. China
ABSTRACT
The morphological features were identified by TEM and total exosome protein concentration was determined by the BCA Protein assay. Under the electron microscope, it was observed that Bactrian camel milk exosomes were a typical vesicle enrichment with a large bilayer lipid membrane structure and the size of 30-200 nm round or oval membranous vesicles in the shape of a cup holder. The centre was evenly dyed black, the film was lightly dyed white and the edges were clear. The total protein concentration of the exosome extract measured by the BCA Protein assay was 18.6404 ± 1.7297 μg/μL. The study provided new ideas for exploring the biological function and principle of action of biological nano-vehicles in Bactrian camel milk.
Key words: Bactrian camel, exome, milk, TEM
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