Camels and Camelids

GENOTYPING OF S. aureus OBTAINED FROM SKIN WOUNDS OF CAMEL (Camelus dromedarius) BASED ON CAPK GENES

Journal Edition: December 2016
Article DOI: 10.5958/2277-8934.2016.00039.4
Published On: 03-09-2018 19:04

Vikas Khichar1, Swati Ruhil2, Vijayata Choudhary3, M.K. Khyalia4 and A.K. Kataria
Apex Centre for Animal Disease Investigation, Monitoring and Surveillance, College of Veterinary and Animal Science,
Rajasthan University of Veterinary and Animal Sciences, Bikaner 333001, India
1Government Veterinary Hospital, Dhab Dhani, Haryana, India
2Department of Veterinary Gynaecology and Obstetrics, 3Department of Veterinary Parasitology,
College of Veterinary and Animal Science, Bikaner 333001, Rajasthan
4Government Veterinary Hospital, Gothtra, Rajasthan, India

ABSTRACT

Staphylococcus aureus is an important pathogen associated with several clinical infections in humans and animals. The present study was carried out to evaluate S. aureus for their 2 major capsular polysaccharide genes namely cap5k and cap8k. Twenty isolates of S. aureus were obtained from skin wounds of camels in Bikaner, Rajasthan which were first identified by conventional cultural and biochemical properties and then confirmed by ribotyping based on 23S rRNA gene segment. All the isolates were processed to detect presence of cap5k and cap8k gene by PCR. The results demonstrated that 18 (90%) isolates were typable of which 55% S. aureus isolates possessed cap5k and 35% possessed cap8k whereas 10% isolates were not typable for any of these 2 genes.
Keywords: Camel, capsular polysaccharide, cap5k and cap8k genes, Staphylococcus aureus