Camels and Camelids

GENETIC AND DEMOGRAPHIC BOTTLENECK ANALYSIS OF MALVI CAMEL BREED BY MICROSATELLITE MARKERS

Journal Edition: June 2015
Article DOI: 10.5958/2277-8934.2015.00002.8
Published On: 03-09-2018 19:06

Sushma Prasad1,2, Sharique A. Ali1 and Vijh R.K.2
1Department of Biotechnology, Saifia Science College, Bhopal-462001
2National Bureau of Animal Genetic Resources, Karnal, Haryana

ABSTRACT

The study included genetic and demographic bottleneck analysis of Malvi camel breeds by microsatellite markers using 29 loci. The genetic diversity of a population of Malvi camel was evaluated. Multilocus genotype data on 29 microsatellites were generated on 138 Malvi camels. A total of 240 alleles were observed. The mean observed heterozygosity in camel population for all the 29 loci was found to be 0.6041 ± 0.3256 while the mean expected heterozygosity was 0.5978 ± 0.1983. The statistical values for within locus k-test obtained for the Malvi camel was 18 loci with negative values which signifies population expansion. The inter locus g-test revealed a value of 2.3557. This test also revealed that Malvi camel population has undergone population expansion. Three tests viz; Sign test (Non-parametric), Standardised Differences test (Parametric), Wilcoxon test and Qualitative graphical method based on the allele frequency spectra reflects that the population had not undergone bottleneck.
Key words: Bottleneck, genetic diversity, malvi camel, microsatellite marker