Camels and Camelids

EFFECTIVENESS EVALUATION OF RECOMBINANT ANTIGEN rCPI FOR iELISA DETECTION OF CAMEL PARABRONEMIASIS

Journal Edition: December 2021
Article DOI: 10.5958/2277-8934.2021.00043.6
Published On: 13-12-2021 21:09

Yu Wang1*, Chenchen Feng1*, Chunxia Liu2, Jianyun LI3 and Wenlong Wang1#
1College of Veterinary Medicine, 2College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018,
China Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,
Ministry of Agriculture, P.R. China, Hohhot 010018, China
3Inner Mongolia Comprehensive Centre for Disease Control and Prevention, Hohhot 010031, China

ABSTRACT

Parabronemiasis is a serious parasitic disease in ruminants and a major parasitic nematode in camels. There is still no ideal method for diagnosing the disease when the hosts are alive. In order to screen the antigen of P. skrjabini for establishing a serological diagnostic method for camel parabronemiasis, in this study, we amplified and cloned the gene encoding cysteine protease inhibitor (cpi) of P. skrjabini by RT-PCR and constructed the expression plasmid pET-cpi, which was then transferred into Escherichia coli BL21 (DE3) to obtain the recombinant protein rCPI. The purified recombinant protein was used as the coating antigen to establish an indirect ELISA diagnostic method for parabronemiasis. A total of 140 sera collected from camels in Inner Mongolia were tested. A recombinant cpi protein rCPI with a size of 20.2 kDa was obtained, which can specifically bind to IgG in the serum of camels infected with P. skrjabini. An iELISA method for the detection of parabronemiasis was established with good specificity. The positive rate of 140 camel sera was 84.3% (118/140), indicating that rCPI iELISA can be used as a serological diagnostic method for camel parabronemiasis.
Key words: Camels, cysteine protease inhibitor, Parabronema skrjabini, Parabronemiasis, serological diagnosis