Journal Edition: December 2020
Article DOI: 10.5958/2277-8934.2020.00035.1
Published On: 02-01-2021 11:40
Bin Yang, Huar Bao, Xiaohu Wang, Tianjian Deng, Haobo Li, Yuye Fu, Xiaojing Xu and Demtu Er
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China; Key Laboratory of Clinical Diagnosis
and Treatment Technology in Animal Disease, Ministry of Agriculture, P.R. China, Hohhot, 010011, China
The purpose of this study was to reveal the molecular mechanism that regulates fat metabolism in hump of Bactrian camel. The subcutaneous fat (SF), greater omentum (GOM) and hump fat (HF) of Bactrian camel were sequenced by RNA sequencing, and the differentially expressed genes of fat tissue in 3 different parts at transcriptome level were obtained by paired comparison. Genes related to fat metabolism were screened out, among which HF vs. GOM group had the largest number of different genes related to fat metabolism. Some genes such as SCD, FASN, ACACA, ADIPOQ, PLIN1, PLIN4, LPL and HSL were highly expressed in HF. These genes may play an important role in maintaining the energy homeostasis of Bactrian camels in the absence of food. Signaling pathway analysis revealed a number of pathways involved in fat metabolism including AMPK signaling pathway, PPAR signaling pathway, Adipocytokine signaling pathway, ECM-receptor interaction and regulation of lipolysis in adipocytes. These data suggests HF has a greater capacity to modulate the release and storage of triglycerides in adipocytes than GOM.
Key words: Adipose, Bactrion camel, RNA sequence, transcriptome