Curriculum Vitae - Nisar Ahmad Wani (Member)

• Doctor of Philosophy (Ph. D.) from GB Pant University of Agriculture and Technology, India (1999 to 2003).
• Master of Veterinary Sciences (1993 to 1996) from SK university of Agricultural Sciences and Technology, Srinagar, India,
• Bachelor of Veterinary Sciences and Animal Husbandry (B. V. Sc. & A. H.), from SK university of Agricultural Sciences and Technology, Srinagar, India (1986 to 1992).

October 2014 – till date

Scientific Director, Reproductive Biotechnology Center, Dubai, UAE.

www.reprobiotech.ae

1. March 2005 – October 2014

Head of Reproductive Biology Laboratory, Camel Reproduction Centre, Dubai, UAE.

1. April 2003 – March 2005

Sr. Scientist, Central Veterinary Research Laboratory, Dubai, UAE.

1. Feb 1998 – April 2003

Assistant Professor Cum Junior Scientist, SK University of Agricultural Sciences and Technology, Srinagar, India.

1. Feb 1992 – Feb 1998

Senior Technical Assistant, SK University of Agricultural Sciences and Technology, Srinagar, India

• Produced World’s First Cloned Camelid, a dromedary camel named “Injaz”, from ovarian cells of a slaughtered animal. Biology of Reproduction.  82, 373–379. https://doi.org/10.1095/biolreprod.109.081083
• Produced World’s First Cloned Bactrian Camel by interspecies Somatic Cell Nuclear Transfer (iSCNT), using dromedary camel as the source of ooplasm as well as surrogate to carry the pregnancy to term.

PLOS ONE12(5): e0177800. https://doi.org/10.1371/journal.pone.0177800

• First report of embryo production by intra-cytoplasmic sperm injection (ICSI) in camels. DOI: 10.1016/j.theriogenology.2018.02.004
• First report of applying and optimizing the technique of ultrasound guided transvaginal ovum pick-up (OPU) in camels. Theriogenology, 74; 436–442. DOI: 10.1016/j.theriogenology.2010.02.026

• First report to study the kinetics of in vivo oocyte maturation in camels. (Ultrasonographic-guided retrieval of in vivo matured oocytes after super-stimulation in dromedary camel (Camelus dromedarius). Theriogenology, 74; 436–442.
• First report of successful liquid storage of epididymal spermatozoa collected from slaughtered camels up to 8 days and production of embryos by IVF from such semen. Animal Reprod Sci. 111; 69–79. DOI: 10.1016/j.anireprosci.2008.02.005
• First report on storage of ovaries and kinetics of oocyte maturation in camels. Theriogenology 64:75-85. DOI: 10.1016/j.theriogenology.2004.11.009
• First report of embryo (blastocyst) production from cryopreserved immature oocytes in buffalo. Theriogenology 61: 831-842).
• First report of successful cryopreservation of ovarian tissue in buffalo. Ph D Thesis. G.B. Pant University of Agriculture and Technology, Pantnagar, India).

1. Wani, NA (2021). In vitro embryo production (IVEP) in camelids: Present status and future perspectives. Reproductive Biology, Volume 21, Issue 1, 2021, 100471. https://doi.org/10.1016/j.repbio.2020.100471.
2. Wani NA., Kumar K. P. (2021). Telomere length in cloned camels produced by somatic cell nuclear transfer is not different from that in their naturally produced counterparts. Reproduction, Fertility and Development 33, 114-114. https://doi.org/10.1071/RDv33n2Ab14
3. Wani NA, Hong SB. Effect of roscovitine pretreatment on in vitro maturation of oocytes and their subsequent developmental after chemical activation in dromedary camel (Camelus dromedarius). Theriogenology, Volume 157,2020, Pages 176-180, https://doi.org/10.1016/j.theriogenology.2020.07.025.
4. Vettical B, Hong SB, Umer, MA and Wani NA. Comparison of pregnancy rates with transfer of in vivo produced embryos derived using multiple ovulation and embryo transfer (MOET) with in vitro produced embryos by somatic cell nuclear transfer (SCNT) in the dromedary camel (Camelus dromedarius). Animal Reproduction Science 2019 209:106132.DOI: 10.1016/j.anireprosci.2019.106132
5. Wani NA, Hong S, Vettical BS (2018). Cytoplast source influences development of somatic cell nuclear transfer (SCNT) embryos in vitro but not their development to term after transfer to synchronized recipients in dromedary camels (Camelus dromedarius). Theriogenology 118:137-143. 
6. Wani NA, Hong SB (2018). Intracytoplasmic sperm injection (ICSI) of in vitro matured oocytes with stored epididymal spermatozoa in camel (Camelus dromedarius): Effect of exogenous activation on in vitro embryo development. Theriogenology 8; 113: 44-49.
7. Wani NA, Hong SB (2018). Source, treatment, and type of nuclear donor cells influences in vitro, and in vivo development of embryos cloned by Somatic Cell Nuclear Transfer in camel (Camelus dromedarius). Theriogenology 15; 106: 186-191.
8. Wani NA, Vettical BS, Hong SB (2017). First cloned Bactrian camel (Camelus bactrianus) calf produced by interspecies somatic cell nuclear transfer: A step towards preserving the critically endangered wild Bactrian camels. PLOS ONE 12(5): e0177800.  
9. Wani NA, KM Morton, M Billah, Skidmore JA (2011). Biophysical and biochemical characteristics of ejaculated dromedary camel (Camelus dromedarius) and llamas (Llama glama) semen. J Camel Practice and Research 18, 97-102.
10. Wani NA, Wernery U, Hassan FAH, Wernery R, Skidmore JA (2010). Production of the First Cloned Camel by Somatic Cell Nuclear Transfer. Biology of Reproduction 82, 373–379.
11. Wani, NA, Wernery U (2010). Effect of different protein supplementations and epidermal growth factor on in vitro maturation of dromedary camel (Camelus dromedarius) oocytes. Reproduction in domestic animals 45, e189–e193
12. Wani NA, Skidmore JA (2010). Ultrasonographic-guided retrieval of in vivo matured oocytes after super-stimulation in dromedary camel (Camelus dromedarius). Theriogenology 74; 436–442
13. Kinne J, Wani N A, Wernery U, Peters J and Knospe C (2010). Is There a Two-Humped Stage in the Embryonic Development of the Dromedary? Anatomia, Histologia, Embryologia doi: 10.1111/j.1439-0264.2010. 01011.
14. Wani NA (2009). In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4⁰C. Animal Reprod Sci. 111; 69–79.
15. Wani NA, Billa M, Skidmore JA (2008). Studies on liquefaction and storage of ejaculated dromedary camel (Camelus dromedarius) semen. Animal Reprod Sci.  109; 309–318.
16. Wani NA (2008). Chemical activation of in vitro matured dromedary camel (Camelus dromedarius) oocytes: optimization of protocols. Theriogenology 69; 5: 591-602.
17. Wani NA and Nowshari MA (2005). Kinetics of nuclear maturation and effect of holding ovaries at room temperature on in vitro maturation of camel (Camelus dromedarius) oocytes. Theriogenology 64:75-85.